This system is ideal for synthesizing primers of various lengths as well as complex modified oligonucleotides plus dye labeled primers and probes.
Oligo synthesis machine price.
The abi 394 4 columns synthesizer is an economical versatile medium throughput platform that synthesizes dna oligonucleotides with fast cycle times low reagent consumption.
New and used dna synthesizers for sale and auction at labx.
The software will automatically adjust reagent delivery according to the scale of the synthesis.
All oligos are synthesized in parallel and the final product is ready for post synthesis treatment in a 96 titre format.
An additional purification cost per oligo for hplc non modified and hplc ce purified oligos.
Whereas enzymes synthesize dna and rna only in a 5 to 3 direction chemical.
Detailed synthesis information can be quickly viewed with the ability to start and stop synthesis at anytime.
These oligos are delivered in 96 well plates and concentration normalized at 50 µm in 200 µl or lyophilized.
Oligonucleotide length sequence gc content and coupling efficiencies can vary the yield of nmole amounts post synthesis.
When ordering custom oligos remember that the scale of synthesis 25 nmol 50 nmol 200 nmol 1 µmol or 10 µmol is the starting point for synthesis not the guaranteed final amount.
We do not rely on third party manufacturers for the machines and chemical reagents used for synthesis allowing us to easily refine equipment and reagents as necessary.
Remember that the synthesis scale 25 nmol 100 nmol etc refers to the starting material for oligo synthesis and not the final yield.
Oligonucleotide synthesis is the chemical synthesis of relatively short fragments of nucleic acids with defined chemical structure the technique is extremely useful in current laboratory practice because it provides a rapid and inexpensive access to custom made oligonucleotides of the desired sequence.
Can be upgraded to make 96 and 192 oligos in parallel.
Synthesis can range from 1 oligo to 768 oligos in a single run while scaling from 1 nmole to 2 umole.
Oligo length sequence gc content and coupling efficiencies alter the final nmol yield.
10 oligos 20 mer in less than 1 5 hours.
Based on an oligo length of 30 bases.
The use i want to do is self synthesis of oligo for general purpose i am spending actually 15 000 o more in oligo synthesis but i am asking if home synthesis could be cheaper any feedback will.
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